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ZytoDot SPEC TOP2A Probe

Ref : C-3021-400 - Nº Test : 40 - Format : 0,4 ml
Manufacturer: ZytoVision
Description

Background

The ZytoDot ® SPEC TOP2A Probe is designed for the detection of TOP2A deletions and gene amplifications in formalin-fixed, paraffin-embedded tissue sections or cell samples. The TOP2A (topoisomerase II alpha) gene is located in the chromosomal region 17q21-17q22 and encodes for a 170 kDa DNA topoisomerase which controls and alters the topologic state of DNA during replication, transcription, and chromosome segregation. TOP2A gene copy number changes are frequently observed in the majority of HER2/neu amplified primary breast tumors as well as in other human malignancies without simultaneous HER2/neu amplification e.g. acute lymphoblastic leukemias, gastric and bladder carcinomas. Recent data suggests that amplification and deletion of the TOP2A gene locus may account for relative chemosensitivity or resistance to TOP2A inhibitor therapy, respectively. Thus, determination of the TOP2A status may predict benefit from adjuvant anthracyclines in HER2 positive breast cancer.

 

References

Arriola E, et al. (2007) Breast Cancer Res Treat 106: 181-9.
Järvinen TA & Liu ET (2006) Curr Cancer Drug Targets 6: 579-602.
Järvinen TA, et al. (2000) Am J Pathol 156: 839-47.
Tanner M, et al. (2006) J Clin Oncol 24: 2428-36.
Tewey KM, et al. (1984) Science 226: 466-8.
Tsai-Pflugfelder M, et al. (1988) Proc Nat Acad Sci 85: 7177-81.
Wang JC (1996) Annu Rev Biochem 65: 635-92.

 

Probe Description

The ZytoDot ® SPEC TOP2A Probe is a Digoxigenin-labeled clone contig specific for the TOP2A gene at 17q21-22, processed by the unique ZytoVision ® Repeat Subtraction Technique resulting in advanced specificity and less background.

 

Results

In normal cells, two distinct dot-shaped signals per nucleus will be observed. Nuclei with amplification of the TOP2A gene locus or polysomy of chromosome 17 will show multiple dots or large signal clusters. Deletion of the TOP2A gene results in one or no signal per nucleus.

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