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ZytoDot 2C SPEC PTEN/CEN 10 Probe

Ref : C-3053-400 - Nº Test : 40 - Format : 0,4 ml
Manufacturer: ZytoVision
Description

Background

The ZytoDot ® 2C SPEC PTEN/CEN 10 Probe is designed for the detection of PTEN deletions frequently observed in many tumor types, including renal, melanoma, endometrial, breast, prostate, lung, bladder, and thyroid cancer but also in hematological neoplasms.
The tumor suppressor gene PTEN (phosphatase and tensin homolog deleted on chromosome ten), often referred to as MMAC1 (mutated in multiple advanced cancers 1), is located on 10q23.3 and encodes a 47 kDa dual-specificity phosphatase that has both lipid and protein phosphatase activity. Its inactivation results in constitutive activation of the PI3K/AKT pathway and in subsequent increase in protein synthesis, cell cycle progression, migration, and survival. Deletions affecting the long arm of chromosome 10 have been detected in 30 to 50% of early and advanced stage sporadic melanomas and about 40 to 70% of prostate cancers. In both tumor entities loss of PTEN has been associated with poor clinical outcome. Currently, several drugs targeting the PI3K/AKT pathway for the therapy of solid tumors have entered clinical trials.

 

References

Dahia PLM, et al. (1999) Hum Mol Genet 8:185-93.
Healy E, et al. (1998) Oncogene 16: 2213-18.
Li J, et al. (1997) Science 275: 1943-47.
Swoboda A, et al. Genes Chromosomes Cancer 50: 680-8.
Weng LP, et al. (2001) Hum Mol Genet 10: 599-604.
Yoshimoto M, et al. (2006) Cancer Genet Cytogenet 169:128-37.
Yoshimoto M, et al. (2007) Br J Cancer 97:678-85.
* Using 10 μl probe solution per test. only available in certain countries. All other countries research use only! Please contact your local dealer for more information.
Prod.

 

Probe Description

The ZytoDot ® 2C SPEC PTEN/CEN 10 Probe is a mixture of a Dinitrophenyllabeled CEN 10 probe specific for the alpha satellite centromeric region of chromosome 10 (D10Z1) and a Digoxigeninlabeled probe specific for the PTEN gene at 10q23.

 

Results

In a normal interphase nucleus, using the ZytoDot ® 2C CISH Implementation Kit two red (CEN 10) and two green (PTEN) signals are expected. In a cell with a deletion of the PTEN gene locus a reduced number of green signals will be observed. Deletions affecting only parts of the PTEN gene might result in normal signal pattern with green signals of reduced size.

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