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ZytoDot 2C SPEC MDM2/CEN 12 Probe

Ref : C-3049-100 - Nº Test : 10 - Format : 0,1 ml
Manufacturer: ZytoVision
Description

Background

The ZytoDot ® 2C SPEC MDM2/CEN 12 Probe is designed for the simultaneous detection of MDM2 and centromere 12 in formalin-fixed, paraffin-embedded tissue sections or cell samples.
The MDM2 (mouse double minute 2) gene is located in the chromosomal region 12q14.3-12q15 and encodes for an E3 ubiquitin ligase which acts as a major negative regulator of the tumor suppressor p53.
MDM2 gene amplifications are found in more than 10% of human tumors. Due to the amplification of the respective chromosomal region, MDM2 is overexpressed in many human tumors such as soft tissue sarcomas, osteosarcomas, gliomas, NSCLC, gastric and breast carcinomas.
Well-differentiated liposarcomas (WDLPS), the most common soft tissue tumors in adults, are characterized by the amplification of 12q-derived chromosomal material, harboring the MDM2 oncogene while lipomas show balanced translocations involving 12q13-15. Accordingly, detection of the 12q14-15 amplification is regarded as a valuable tool for the differential diagnosis between well-differentiated liposarcomas and lipomas. Furthermore, detection of the MDM2 amplification might have prognostic relevance in gastrointestinal stromal tumors (GIST), the most common primary mesenchymal tumor of the gastrointestinal tract.

 

References

Flanagan A ,et al. (2010) Skeletal Radiol 39: 213-24.
Kashima T, et al. (2012) Mod Pathol 25: 1384-96.
Korcheva VB, et al. (2012) Appl Immunhistochem Mol Morphol 19: 119-25.
Larousserie F, et al. (2012) Eur J Radiol [Epub ahead of print].
Luan SL, et al. (2010) J Pathol 222: 166-79.
Momand J, et al. (1992) Cell 69: 1237-45.
Oliner JD, et al. (1992) Nature 358: 80-3.
Pedeutour F, et al. (1994) Genes Chromosomes Cancer 10: 85-94.
Pedeutour F, et al. (2004) Bull Cancer 91: 317-23.
Poaty H, et al. (2012) PLoS ONE 7: e29426.
Toledo F & Wahl GM (2006) Nat Rev Cancer 6: 909-23.
Tornillo L, et al. (2005) Lab Invest 85: 921-31.
Vassilev LT (2007) Trends Mol Med 13: 23-31.

 

Probe Description

The ZytoDot ® 2C SPEC MDM2/CEN 12 Probe is a mixture of a Digoxigenin-labeled probe specific for MDM2 gene at 12q 14.3-12q15 and a Dinitrophenyl-labeled CEN 12 probe specific for the alpha satellite centromeric region of chromosome 12 (D12Z3).

Results

In a normal interphase nucleus,using the ZytoDot ® 2C CISH Implementation Kit two red and two green signals are expected.
In a cell with amplification of the MDM2 gene locus, multiple copies of the green signal or green signal clusters will be observed.

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