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ZytoDot 2C SPEC ERG Break Apart Probe

Ref : C-3058-400 - Nº Test : 40 - Format : 0,4 ml
Manufacturer: ZytoVision


The ZytoDot ® 2C SPEC ERG Break Apart Probe is designed to detect aberrations involving the ERG gene at 21q22.2 frequently found in prostate cancers.
ERG (ETS-related gene) rearrangements have been observed in 40-60% of prostate cancers identified via prostatespecific antigen (PSA) screening. The most common aberration affecting ERG is the interstitial deletion of about 3 Mb at the chromosomal region 21q22 found in 90% of the cases. This deletion leads to the fusion of the hormonally regulated promoter of the TMPRSS2 (transmembrane protease, serine 2) gene to the coding region of ERG, resulting in overexpression of the ERG transcription factor. However, about 10% of the ERG rearranged prostate cancer cases show alternative fusions, as e.g. SLC45A3-ERG or NDRG1-ERG.
Several studies detected associations of ERG rearrangements with histomorphologic features as well as characteristic copy number gains, and gene expression signatures, defining a distinct sub-class of prostate cancers with unfavorable prognosis.
Hence, the evaluation of the ERG rearrangement status in tissue or urine samples by CISH might be of diagnostic and prognostic relevance.
EWSR1-ERG gene fusions present in about 10% of patients with Ewing sarcoma may result from complex genomic rearrangements and may therefore not be detected by CISH analysis or may result in a nonclassical translocation signal pattern.



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Perner S, et al. (2006) Cancer Res 66: 8337-41.
Pflueger D, et al. (2009) Neoplasia 11: 804-11.
Tomlins SA, et al. (2005) Science 310: 644-8.


Probe Description

The ZytoDot ® 2C SPEC ERG Break Apart Probe is a mixture of a Digoxigeninlabeled and a Dinitrophenyl-labeled probe hybridizing to the long arm of chromosome 21. The DNP- labeled probe hybridizes proximal to the ERG gene breakpoint region at 21q22.13, the DIG-labeled probe hybridizes distal to the ERG gene breakpoint region at 21q22.2.



In an interphase nucleus of a normal cell lacking an aberration involving the 21q22 band, using the ZytoDot ® 2C CISH Implementation Kit, two red/green fusion signals are expected representing the two normal (non-rearranged) 21q22.2 loci.
A 21q22 locus affected by a 21q22.2 deletion resulting in the TMPRSS2-ERG fusion is indicated by the loss of one green signal.
A signal pattern consisting of one red/ green fusion signal, a separate green, and a separate red signal indicates an ERG translocation without involvement of TMPRSS2 (e.g. SLC45A3-ERG).

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