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ZytoDot 2C SPEC ALK Break Apart Probe

Ref : C-3055-400 - Nº Test : 40 - Format : 0,4 ml
Manufacturer: ZytoVision
Description

Background

The ZytoDot ® 2C SPEC ALK Break Apart Probe is designed to detect rearrangements involving the chromosomal region 2p23
harboring the ALK (anaplastic lymphoma receptor tyrosine kinase, a.k.a. CD246) gene.
ALK encodes a transmembrane receptor tyrosine kinase. This gene exerts characteristic oncogenic activities through fusion to several gene partners or mutations both in hematopoietic and non-hematopoietic solid tumors.
Translocations affecting the ALK gene locus are frequently found in anaplastic large cell lymphoma (ALCL), an aggressive non-Hodgkin lymphoma arising from Tcells.
The most frequent translocation t(2;5) results in a fusion with the NPM1 (nucleophosmin a.k.a. nucleolar phosphoprotein B23, numatrin) gene located on chromosome 5q35. This rearrangement results in a NPM1/ALK fusion protein, which is constitutively activated through autophosphorylation, and that in turn mediates malignant cell transformation by activating downstream effectors like e.g. STAT3.
Additionally, inversions affecting the ALK gene located on the short arm of chromosome 2 [inv(2)(p21p23)] have been frequently detected in non-small cell lung cancer (NSCLC) and lead to the formation of EML4-ALK fusion transcripts.
ALK kinase targeted therapies may represent a very effective therapeutic strategy in NSCLC patients carrying EML4-ALK rearrangements.

 

References

Inamura K, et al. (2009) Mod Pathol 22: 508-15.
Koivunen JP, et al. (2008) Clin Cancer Res 14: 4275-83.
Martelli MP, et al. (2009) Am J Pathol 174: 661-70.
Palmer RH, et al. (2009) Biochem J 420: 345-61.
Perner S, et al. (2008) Neoplasia 10: 298-302.
Rodig SJ, et al. (2009) Clin Cancer Res 15: 5216-23.
Sasaki T, et al. (2010) Eur J Cancer 46: 1773-80.
Zhang Q, et al. (2007) Nat Med 11:1341-8.

 

Probe Description

The ZytoDot ® 2C SPEC ALK Break Apart Probe is a mixture of a Digoxigeninlabeled probe and a Dinitrophenyl-labeled probe hybridizing to the 2p23 band. The Digoxigenin-labeled probe hybridizes proximal to the ALK gene at 2p23, the Dinitrophenyl-labeled probe hybridizes distal to the ALK gene at 2p23.

 

Results

In an interphase nucleus of a normal cell lacking a translocation involving the 2p23 band, using the ZytoDot ® 2C CISH Implementation Kit, two red/green fusion signals are expected representing two normal (non-rearranged) 2p23 loci. A signal pattern consisting of one red/green fusion signal, one red signal, and a separate green signal indicates one normal 2p23 locus and one 2p23 locus affected by a translocation or inversion.

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