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ZytoDot SPEC MDM2 Probe

Ref : C-3012-400 - Nº Test : 40 - Format : 0,4 ml
Manufacturer: ZytoVision
Description

Background

The ZytoDot ® SPEC MDM2 Probe is designed for the detection of MDM2 gene amplifications found in more than 10% of human tumors. The MDM2 (murine double minute 2) gene is located in the chromosomal region 12q14.3-12q15 and encodes for an E3 ubiquitin ligase which acts as a major negative regulator of the tumor suppressor p53. Due to the amplification of the respective chromosomal region, MDM2 is overexpressed in many human tumors such as soft tissue sarcomas, osteosarcomas, gliomas, NSCLC, gastric and breast carcinomas. Well-differentiated liposarcomas (WDLPS), the most common soft tissue tumors in adults, are characterized by the amplification of 12q-derived chromosomal material, harboring the MDM2 oncogene while lipomas show balanced translocations involving 12q13-15. Accordingly, detection of the 12q14-15 amplification is regarded as a valuable tool for the differential diagnosis between welldifferentiated liposarcomas and lipomas. Furthermore, detection of the MDM2 amplification might have prognostic relevance in gastrointestinal stromal tumors (GIST), the most common primary mesenchymal tumor of the gastrointestinal tract.

 

References

Korcheva VB, et al. (2011) Appl Immunhistochem Mol Morphol 19: 119-25.
Larousserie F, et al. (2012) Eur J Radiol [Epub ahead of print].
Luan SL, et al. (2010) J Pathol 222: 166-79.
Momand J, et al. (1992) Cell 69: 1237-45.
Oliner JD, et al. (1992) Nature 358: 80-3.
Pedeutour F, et al. (1994) Genes Chromosomes Cancer 10: 85-94.
Pedeutour F, et al. (2004) Bull Cancer 91: 317-23.
Poaty H, et al. (2012) PLoS ONE 7: e29426.
Toledo F & Wahl GM (2006) Nat Rev Cancer 6: 909-23.
Tornillo L, et al. (2005) Lab Invest 85: 921-31.
Vassilev LT (2007) Trends Mol Med 13: 23-31.

 

Probe Description

The ZytoDot ® SPEC MDM2 Probe is a Digoxigenin-labeled clone contig specific for the MDM2 gene region at 12q14.3- 12q15, processed by the the unique ZytoVision ® Repeat Subtraction Technique resulting in advanced specificity and less background.

 

Results

In normal cells, two distinct dot-shaped signals per nucleus will be observed. Nuclei with amplification of the MDM2 gene locus or polysomy of chromosome 12 will show multiple dots or large signal clusters.

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