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Anti-human IDH1 R132H (Dianova GmbH)

 

Astrocytoma & Oligodendroglioma Tumor Cell Marker (Dianova GmbH Ref: DIA-H09)  Imagen 1

The antibody clone H09 is the first commercially available antibody, that specifically stains single cells carrying the IDH1 R132H point mutation in FFPE brain tumor sections.

Clone H09: The first commercially available antibody that specifically reacts with the IDH1 R132H point mutation in formalin-fixed paraffin-embedded brain tumor specimen!

Heterozygous point mutations of isocitrate dehydrogenase (IDH) 1 codon 132 occur in approximately 70% of astrocytomas and oligodendroglial tumors. The high frequency and distribution of the IDH1 R132H mutation among specific brain tumor entities allow the highly sensitive and specific discrimination of various tumors by immunohistochemistry, such as anaplastic astrocytoma from primary glioblastoma or diffuse astrocytoma WHO grade II from pilocytic astrocytoma or ependymoma. Noteworthy is the discrimination of the infiltrating edge of tumors with IDH1 mutation from reactive gliosis. The antibody clone H09 is highly useful for brain tumor classification and for the detection of single infiltrating tumor cells.

Immunohistochemistry of human IDH1 R132H in standard formalin-fixed paraffin-embedded tissue sections

Pictures courtesy of Prof. Dr. A. von Deimling, Department of Neuropathology, University of Heidelberg / Clinical Cooperation Unit Neuropathology, German Cancer Research Center (DKFZ), Heidelberg

References

 

1. Capper et al. (2009) Characterization of R132H mutation-specific IDH1 antibody in brain tumors. Brain Pathol. (Epub ahead of print)

2. Capper et al. (2009) Monoclonal antibody specific for IDH1 H132R mutation. Acta Neuropathol. 118(5): 599-601

3. Andrulis M, Capper D, Luft, T, Hartmann C, Zentgraf H, von Deimling A. Detection of isocitrate dehydrogenase 1 mutation R132H in myelodysplastic syndrome by mutation-specific antibody and direct sequencing. Leuk Res. 2010 Mar 11 (Epub ahead of print)

 

Figure 1 IDH1 R132H AK - 165 - OAIII - solider Tumor d Figure 2 IDH1 R132H AK - 277 - AIII - Infiltrationszone h Figure 3 IDH1 R132H AK - 148 - AIII - Infiltrationszone i
Strong reaction of antibody clone H09 with IDH1 R132H mutated diffuse astrocytoma (top) but not with wild type tumor (bottom). Double staining of GFAP (glial fibrillary acidic protein, red) and clone H09 (brown) of oligodendroglioma infiltration zone demonstrating specific labelling of tumor cells but not of GFAP positive reactive astrocytes. Strong reaction of antibody clone H09 in tumor center of anaplastic oligoastrocytoma.
 Figure 4 IDH1 R132H AK - 289 - OIII - Infiltrationszone f  Figure 5 08-1877 40x  Figure 6 IDH1 R132H AK - AII pos neg Kombi IVa
 Infiltration zone of anaplastic astrocytoma with specific labelling of infil-trating glioma cells by antibody clone H09 Identification of single tumor cells in white matter distant from tumor center with antibody clone H09. Cortex infiltrated by oligo-dendroglioma with specific labelling of tumor cells by antibody clone H09.
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